EvaGreen Supermix

SsoFast EvaGreen Supermix utilizes our patented Sso7d fusion protein technology* for high performance in a wide range of qPCR applications. The dsDNA-binding protein Sso7d stabilizes the polymerase-template complex, increasing processivity and reducing reaction times compared with conventional DNA polymerases without affecting PCR sensitivity, efficiency, or reproducibility. EvaGreen formulation provides increased fluorescence compared to SYBR Green.

Key Features and Benefits:

• Fast qPCR results and high performance — instant activation and rapid polymerization kinetics generate exceptional qPCR results in less than 30 min
• Minimal PCR inhibition — increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility
• Single-copy detection — provides high sensitivity with amplification and detection from a single copy of a target gene
• Robust discrimination and reproducibility — achieves efficient discrimination and reliable quantification from 1.33-fold serial dilutions of template

Applications and Uses:

• Dye-based qPCR detection
• Gene expression analysis
• Genotyping studies

Manufacturer: BIO-RAD LABORATORIES INC.

Package Size: 50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions