Description
MyTaqâ„¢ is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer systems that deliver very high-yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors.
Robust amplification of GC-rich human genomic DNA (61% GC content) MyTaq was compared with DNA polymerases from other suppliers for the amplification of a 450 bp fragment of the human myc gene. Decreasing amounts of human genomic DNA were used as a template (1 µg, 200 ng, 100 ng, 50 ng, 25 ng, and 12.5 ng, lanes 1-6 respectively, HyperLadder 1kb (M)). MyTaq delivers higher yield and sensitivity as compared with all five competing products.
