dsDNA Quantitation High Sensitivity Assay Kits

Achieve accurate and precise quantification of dsDNA with Qubit dsDNA HS (High Sensitivity) and Qubit dsDNA BR (Broad Range) Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high-abundance DNA samples and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides. Contaminants, such as salts, solvents, or detergents are well-tolerated.

Quantify dsDNA quickly and easily, even in the presence of contaminants, with the Qubit dsDNA HS (High Sensitivity) and Qubit dsDNA BR (Broad Range) Assay kits. The dsDNA assay kits are designed specifically for use with the Qubit Fluorometer and are highly selective for double-stranded DNA (dsDNA) over RNA. They are intended to be accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL.

Qubit dsDNA HS Assay Kit features:
• Core range (high confidence): 1 ng/mL to 500 ng/mL
• Extended ranges (moderate confidence): 0.5 ng/mL to 1 ng/mL and 500 ng/mL to 600 ng/mL
• Useful for quantitation of PCR products, viral DNA, and samples for subcloning

Qubit dsDNA BR Assay Kit features:
• Core range (high confidence): 0.01 µg/mL to 5 µg/mL
• Extended range (moderate confidence): 5 µg/mL to 10 µg/mL
• Useful for quantitation of genomic and miniprep DNA samples

Both kits provide concentrated assay reagents, dilution buffers, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well tolerated in either assay.

Manufacturer: Invitrogenâ„¢